Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Biochem Biophys Res Commun
; 321(2): 275-9, 2004 Aug 20.
Article
in En
| MEDLINE
| ID: mdl-15358172
ABSTRACT
We have established a transgenic mouse line in which floxed neomycin resistant cassette was inserted between the CAG promoter and EGFP. When these transgenic mice were mated with Cre-expressing transgenic animals, the offspring obtained were fluorescent green. We then established a transgenic mouse line in which EGFP in the above construct was replaced by diphtheria toxin A chain (DT). When the latter transgenic mice were mated with mice expressing Cre restricted to germ cells, we obtained healthy but sterile offspring due to a disruption of germ line cells by DT expression. We predict that this strategy will be useful for the construction of new animal models for human diseases, featuring a variety of missing cell lineages produced by disruption with DT.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Fragments
/
Viral Proteins
/
Gene Expression
/
Cell Lineage
/
Integrases
/
Diphtheria Toxin
/
Disease Models, Animal
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2004
Document type:
Article
Affiliation country: