Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum.
FEMS Microbiol Lett
; 243(2): 505-11, 2005 Feb 15.
Article
in En
| MEDLINE
| ID: mdl-15686855
ABSTRACT
Based on the intergenic sequences of Tri5-Tri6 genes involved in the mycotoxin pathways of Fusarium species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)-chemotypes and a 360 bp sequence of nivalenol (NIV)- chemotypes of Fusarium graminearum. Mycotoxin chemotypes identified by the PCR assays were confirmed by the chemical analyses of HPLC or GC/MS. Further analysis of 364 F. graminearum isolates from 12 provinces of China showed that 310 were DON-chemotypes and 54 were NIV-chemotypes. Sequence analyses revealed that DON-chemotypes display more variations than NIV-chemotypes. This PCR assay could be used to detect mycotoxin-producing Fusarium-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. Also this assay may provide useful alternatives to antibody-based mycotoxin tests.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trichothecenes
/
Fungal Proteins
/
Polymerase Chain Reaction
/
DNA, Ribosomal Spacer
/
Fusarium
/
Mycotoxins
Type of study:
Diagnostic_studies
/
Evaluation_studies
Language:
En
Journal:
FEMS Microbiol Lett
Year:
2005
Document type:
Article