[Effects of rapamycin on Rho-kinase and p27 mRNA expressions in a porcine coronary intimal proliferation model induced by interleukin-1beta].
Zhonghua Xin Xue Guan Bing Za Zhi
; 34(5): 445-9, 2006 May.
Article
in Zh
| MEDLINE
| ID: mdl-16776964
ABSTRACT
OBJECTIVE:
To observe the effects of rapamycin on the expressions of Rho-kinase and p27 mRNA during vascular intimal proliferation in a porcine model of coronary stenosis induced by interleukin-1beta (IL-1beta).METHODS:
The proximal segments of LAD and LCX were wrapped with cotton mesh that had absorbed sepharose bead solution with or without IL-1beta. Selective coronary angiography was performed two weeks later and the animals were killed for collecting the samples for histopathology and RT-PCR analyzing of Rho-kinase and p27 mRNA.RESULTS:
The expressions of Rho-kinase and p27 mRNA could be visualized in normal coronary wall. The expression of Rho-kinase mRNA was significantly enhanced and the expression of p27 mRNA was significantly decreased during the process of intimal proliferation induced by IL-1beta. Rapamycin significantly inhibited the intimal proliferation, reduced the infiltration of inflammatory cells, reduced the expression of Rho-kinase mRNA and increased the expression of p27 mRNA.CONCLUSIONS:
The expression of Rho-kinase mRNA is upregulated and p27 mRNA downregulated in coronary artery stenosis induced by IL-1beta and these effects could be abolished by cotreatment with rapamycin.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tunica Intima
/
Coronary Vessels
/
Sirolimus
/
Interleukin-1beta
/
Rho-Associated Kinases
Limits:
Animals
Language:
Zh
Journal:
Zhonghua Xin Xue Guan Bing Za Zhi
Year:
2006
Document type:
Article
Affiliation country: