A potential role for interleukin-18 in inhibition of the development of Cryptosporidium parvum.
Clin Exp Immunol
; 145(3): 555-62, 2006 Sep.
Article
in En
| MEDLINE
| ID: mdl-16907926
ABSTRACT
Accumulating evidence suggests that intestinal epithelial cells (IECs) constitutively express the immunoregulatory cytokine interleukin (IL)-18. IECs also serve as the host cell for the intracellular parasitic protozoan Cryptosporidium parvum. In the present study, C. parvum infection of a human enterocyte cell-line HCT-8 resulted in increased expression of IL-18 mRNA as measured by quantitative reverse transcription-polymerase chain reaction (RT-PCR). IL-18 protein was detected in control uninfected cells and following infection there was increased expression as measured by enzyme-linked immunosorbent assay (ELISA). Gene expression revealed the presence of the IL-18 receptor subunits not only in cell-lines but also in freshly isolated IECs, suggesting that IL-18-mediated signalling events may contribute to epithelial host defence during infection. Recombinant IL-18 inhibited intracellular development of the parasite in HCT-8 and HT-29 cells. Increased expression of bactericidal antibiotic peptides LL-37 and alpha-defensin 2 by IL-18 in HCT-8 and HT-29 cells may represent one mode of action by which this pluripotent cytokine aids in limiting the development of intracellular pathogens such as C. parvum in the gastrointestinal tract.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cryptosporidium parvum
/
Interleukin-18
/
Enterocytes
/
Cryptosporidiosis
Type of study:
Observational_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Clin Exp Immunol
Year:
2006
Document type:
Article
Affiliation country: