[A method for determining urea by using immobilized urease]. / Metod opredeleniia mocheviny pri pomoshchi immobilizovannoi ureazy.

ABSTRACT

An available method was developed for manual measurements of urea in biological fluids with the use of urease immobilized in sepharose gel. The suggested technique is more reagent-saving (the same reagent may be used for up to 500 times), 3-6-fold more rapid, and twice more sensitive than the routine phenolhypochlorite method with soluble urease. The method is characterized by a high analytical reliability, its results are in high correlation with the soluble urease procedure and with the diacetylmonoxime method (r = 07988 and 0.995, respectively). It is particularly fit for rapid analysis of the urea and convenient for small laboratories.