Polyclonal antibodies mediated immobilization of a peroxidase from ammonium sulphate fractionated bitter gourd (Momordica charantia) proteins.
Biomol Eng
; 24(2): 223-30, 2007 Jun.
Article
in En
| MEDLINE
| ID: mdl-17137839
ABSTRACT
Polyclonal antibody bound Sepharose 4B support has been exploited for the immobilization of bitter gourd peroxidase directly from ammonium sulphate precipitated proteins. Immunoaffinity immobilized bitter gourd peroxidase exhibited high yield of immobilization. IgG-Sepharose 4B bound bitter gourd peroxidase showed a higher stability against heat, chaotropic agents (urea and guanidinium chloride), detergents (cetyl trimethyl ammonium bromide and Surf Excel), proteolytic enzyme (trypsin) and water-miscible organic solvents (propanol, THF and dioxane). The activity of immobilized bitter gourd peroxidase was significantly enhanced in the presence of cetyl trimethyl ammonium bromide and after treatment with trypsin as compared to soluble enzyme.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Peroxidase
/
Coated Materials, Biocompatible
/
Momordica charantia
/
Chemical Fractionation
/
Ammonium Sulfate
/
Antibodies, Monoclonal
Language:
En
Journal:
Biomol Eng
Journal subject:
BIOLOGIA MOLECULAR
/
ENGENHARIA BIOMEDICA
Year:
2007
Document type:
Article
Affiliation country: