SNIP1 is a candidate modifier of the transcriptional activity of c-Myc on E box-dependent target genes.
Mol Cell
; 24(5): 771-783, 2006 Dec 08.
Article
in En
| MEDLINE
| ID: mdl-17157259
ABSTRACT
Using a yeast two-hybrid screen, we found that SNIP1 (Smad nuclear-interacting protein 1) associates with c-Myc, a key regulator of cell proliferation and transformation. We demonstrate that SNIP1 functions as an important regulator of c-Myc activity, binding the N terminus of c-Myc through its own C terminus, and that SNIP1 enhances the transcriptional activity of c-Myc both by stabilizing it against proteosomal degradation and by bridging the c-Myc/p300 complex. These effects of SNIP1 on c-Myc likely contribute to synergistic effects of SNIP1, c-Myc, and H-Ras in inducing formation of foci in an in vitro transformation assay and also in supporting anchorage-independent growth. The significant association of SNIP1 and c-Myc staining in a non-small cell lung cancer tissue array is further evidence that their activities might be linked and suggests that SNIP1 might be an important modulator of c-Myc activity in carcinogenesis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
Proto-Oncogene Proteins c-myc
/
Promoter Regions, Genetic
/
Carcinoma, Non-Small-Cell Lung
/
Intracellular Signaling Peptides and Proteins
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Mol Cell
Journal subject:
BIOLOGIA MOLECULAR
Year:
2006
Document type:
Article