An experimentally derived database of candidate Ras-interacting proteins.
J Proteome Res
; 6(5): 1806-11, 2007 May.
Article
in En
| MEDLINE
| ID: mdl-17439166
ABSTRACT
We used a TAP-tag approach to identify candidate binding proteins for the related Ras family GTPases H-Ras, R-Ras, and Rap1A. Protein complexes were isolated from mouse fibroblasts, and component proteins were identified by a combination of nanoflow HPLC and tandem mass spectrometry. H-Ras was found to associate with numerous cytoskeletal proteins including talin-1. R-Ras and Rap1A each associated with various signaling molecules, many of which are membrane-associated. Thus, we have established the first database of potential Ras interactors in mammalian cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatography, Affinity
/
Ras Proteins
/
Rap1 GTP-Binding Proteins
/
Databases, Protein
Limits:
Animals
Language:
En
Journal:
J Proteome Res
Journal subject:
BIOQUIMICA
Year:
2007
Document type:
Article
Affiliation country: