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Ultrashort electric pulse induced changes in cellular dielectric properties.
Garner, Allen L; Chen, George; Chen, Nianyong; Sridhara, Viswanadham; Kolb, Juergen F; Swanson, R James; Beebe, Stephen J; Joshi, Ravindra P; Schoenbach, Karl H.
Affiliation
  • Garner AL; Bioelectromagnetism Laboratory, Department of Nuclear Engineering and Radiological Sciences, University of Michigan, Ann Arbor, MI 48109, USA; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA. Electronic address: garner@ge.com.
  • Chen G; School of Electronics and Computer Science, University of Southampton, Southampton So17 1bj, UK.
  • Chen N; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA.
  • Sridhara V; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA.
  • Kolb JF; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA.
  • Swanson RJ; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529, USA.
  • Beebe SJ; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA 23510, USA.
  • Joshi RP; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA.
  • Schoenbach KH; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA. Electronic address: kschoenb@odu.edu.
Biochem Biophys Res Commun ; 362(1): 139-144, 2007 Oct 12.
Article in En | MEDLINE | ID: mdl-17706595
The interaction of nanosecond duration pulsed electric fields (nsPEFs) with biological cells, and the models describing this behavior, depend critically on the electrical properties of the cells being pulsed. Here, we used time domain dielectric spectroscopy to measure the dielectric properties of Jurkat cells, a malignant human T-cell line, before and after exposure to five 10ns, 150kV/cm electrical pulses. The cytoplasm and nucleoplasm conductivities decreased dramatically following pulsing, corresponding to previously observed rises in cell suspension conductivity. This suggests that electropermeabilization occurred, resulting in ion transport from the cell's interior to the exterior. A delayed decrease in cell membrane conductivity after the nsPEFs possibly suggests long-term ion channel damage or use dependence due to repeated membrane charging and discharging. This data could be used in models describing the phenomena at work.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Membrane / Electrochemistry Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Biochem Biophys Res Commun Year: 2007 Document type: Article Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Membrane / Electrochemistry Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Biochem Biophys Res Commun Year: 2007 Document type: Article Country of publication: