Efficiency of methacrylate monolithic columns in reversed-phase liquid chromatographic separations.
J Chromatogr A
; 1175(1): 81-8, 2007 Dec 14.
Article
in En
| MEDLINE
| ID: mdl-18001748
ABSTRACT
Butyl-methacrylate-based porous monoliths were prepared inside fused-silica capillaries as reversed-phase separation media for liquid chromatography (LC) and capillary electrochromatography (CEC). During our previous research on methacrylate-based monoliths for reversed-phase separations, we noticed that a separation efficiency of up to 300,000 plates/m can easily be obtained in the CEC mode for unretained compounds. However, the efficiencies for retained compounds were much lower in reversed-phase systems, especially in pressure-driven LC. In this work methacrylate-based columns were prepared and characterized in terms of efficiency and retention in reversed-phase (pressure-driven) LC and in CEC. Much attention has been paid to the mass-transfer mechanism in the stationary phase. Factors that affect the plate heights for specific compounds have been investigated. A possible explanation for the relatively low separation efficiency of retained compounds and suggestions to improve molecular mass transfer are provided.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatography, Liquid
/
Methacrylates
Language:
En
Journal:
J Chromatogr A
Year:
2007
Document type:
Article
Affiliation country: