Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles.
Drug Deliv
; 14(8): 525-9, 2007 Nov.
Article
in En
| MEDLINE
| ID: mdl-18027182
ABSTRACT
The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fibroblast Growth Factor 2
/
Drug Delivery Systems
/
Chitosan
/
Nanoparticles
Language:
En
Journal:
Drug Deliv
Journal subject:
FARMACOLOGIA
/
TERAPIA POR MEDICAMENTOS
Year:
2007
Document type:
Article
Affiliation country: