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Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR.
Frolow, O; Endeward, B; Schiemann, O; Prisner, T F; Engels, J W.
Affiliation
  • Frolow O; Institut für Organische Chemie und Chemische Biologie (OCCB), Goethe-Universität Frankfurt am Main, Max-von-Laue Str. 7, D-60438 Frankfurt am Main, Germany.
Nucleic Acids Symp Ser (Oxf) ; (52): 153-4, 2008.
Article in En | MEDLINE | ID: mdl-18776299
Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligonucleotides / Spin Labels / RNA / Cyclic N-Oxides Language: En Journal: Nucleic Acids Symp Ser (Oxf) Year: 2008 Document type: Article Affiliation country: Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligonucleotides / Spin Labels / RNA / Cyclic N-Oxides Language: En Journal: Nucleic Acids Symp Ser (Oxf) Year: 2008 Document type: Article Affiliation country: Country of publication: