Function of oleic acid on epidermal barrier and calcium influx into keratinocytes is associated with N-methyl D-aspartate-type glutamate receptors.
Br J Dermatol
; 160(1): 69-74, 2009 Jan.
Article
in En
| MEDLINE
| ID: mdl-18808414
ABSTRACT
BACKGROUND:
Unsaturated fatty acids from sebum affect calcium dynamics in epidermal keratinocytes, disrupt the barrier function and induce abnormal keratinization. However, the mechanisms of these effects have not been clarified.OBJECTIVES:
To investigate the function of unsaturated fatty acids in epidermis.METHODS:
Antagonists of calcium channel receptors were applied to mouse skin together with oleic acid. Measurements were made of transepidermal water loss (TEWL), and hyperproliferation was assessed. The effects of the antagonists on calcium influx into cultured normal human keratinocytes and on cytokine production were also evaluated.RESULTS:
N-methyl-d-aspartate (NMDA) receptor antagonists such as MK801 and D-AP5 specifically inhibited the increase in TEWL caused by oleic acid, and suppressed keratinocyte hyperproliferation. These compounds also inhibited the increase in the intracellular concentration of calcium ions induced by oleic acid. MK801 suppressed the production of interleukin-1alpha by keratinocytes induced by oleic acid.CONCLUSIONS:
Unsaturated fatty acids such as oleic acid might function via NMDA receptors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Keratinocytes
/
Calcium
/
Receptors, N-Methyl-D-Aspartate
/
Acne Vulgaris
/
Oleic Acid
Type of study:
Risk_factors_studies
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Br J Dermatol
Year:
2009
Document type:
Article
Affiliation country: