Evaluation of dual priming oligonucleotide-based multiplex PCR for detection of HBV YMDD mutants.
Arch Virol
; 153(11): 2019-25, 2008.
Article
in En
| MEDLINE
| ID: mdl-18836856
ABSTRACT
We evaluated the usefulness of dual priming oligonucleotide (DPO)-based multiplex PCR, Seeplex HBV Lami-DR assay (Seegene Institute of Life Sciences, Seoul, Korea), to detect lamivudine-resistant HBV mutants in a comparison with the use of TRUGENE HBV genotyping and restriction fragment mass polymorphism (RFMP). Sera from 44 chronic hepatitis B patients were analyzed for the presence of mutations at codons 180 and 204 by performing DPO-based multiplex PCR, RFMP, and TRUGENE. The overall concordance rate among the three assays was 40.9% (18/44). Concordance rates between multiplex PCR and RFMP or multiplex PCR and TRUGENE were 61.4% (27/44) and 50.0% (22/44), respectively. In ten patients, multiplex PCR identified additional mutants not found using the other two methods. DPO-based multiplex PCR is a highly sensitive method to identify minor mutant populations and could be a practical tool in the monitoring of lamivudine resistance.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotides
/
Hepatitis B virus
/
Polymerase Chain Reaction
/
DNA Primers
/
Hepatitis B, Chronic
/
Mutation
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
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Male
/
Middle aged
Language:
En
Journal:
Arch Virol
Year:
2008
Document type:
Article
Affiliation country: