Presence of phospholipase C in coated vesicles from bovine brain. Dual regulatory effects of GTP-analogs.
FEBS Lett
; 290(1-2): 22-6, 1991 Sep 23.
Article
in En
| MEDLINE
| ID: mdl-1915878
ABSTRACT
Bovine brain coated vesicles display free calcium-dependent phospholipase C activity. Gpp(NH)p and GTP gamma S inhibited phospholipase C at nanomolar concentrations. Increasing concentrations of Gpp(NH)p and GTP gamma S reversed the inhibitory effects and stimulated phospholipase C activity. Preincubation of coated vesicles with pertussis toxin blocked the poorly-hydrolyzable GTP-analogs' inhibitory effects on phospholipase C. These data indicate that guanine nucleotides exert a dual regulatory control of phospholipase C in coated vesicles and that the inhibitory pathway is mediated by a pertussis toxin-sensitive G-protein.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Type C Phospholipases
/
Brain
/
Coated Pits, Cell-Membrane
/
GTP-Binding Proteins
/
Guanine Nucleotides
Limits:
Animals
Language:
En
Journal:
FEBS Lett
Year:
1991
Document type:
Article
Affiliation country: