Surface changes in adult Fasciola hepatica following treatment in vivo with the experimental fasciolicide, compound alpha.
Parasitol Res
; 105(3): 757-67, 2009 Sep.
Article
in En
| MEDLINE
| ID: mdl-19458965
ABSTRACT
Sheep infected with the triclabendazole-susceptible Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks postinfection. Adult flukes were recovered from the bile ducts at 24, 48, and 72 h post-treatment (p.t.). Changes to the surface morphology of the flukes were assessed using scanning electron microscopy (SEM). Flukes were still active at 24 h p.t. and displayed limited areas of disruption, which were restricted to the oral cone region. At 48 h p.t., a reduced level of motility was observed in approximately 50% of the flukes recovered. Swelling of the tegument was more widespread and was accompanied by blebbing and partial loss of the tegumental covering of the spines. By 72 h p.t., the reduction in motility was greater, and approximately one quarter of the flukes recovered were inactive. In the majority of the flukes examined, the midbody region was marked by a discoloration of the flukes' tissues. This was seen to be due to the loss of the tegumental syncytium. Sloughing extended into the tail region in some specimens and, in the more badly-affected specimens, the basal lamina was breached to expose the underlying musculature. Elsewhere on the body, the tegument that remained was relatively normal, although areas of swelling and blebbing were present. Overall, the results provided information on the time-scale of changes to the surface morphology of the fluke that underpin the efficacy of compound alpha.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sheep Diseases
/
Fasciola hepatica
/
Fascioliasis
/
Antiplatyhelmintic Agents
Limits:
Animals
Language:
En
Journal:
Parasitol Res
Journal subject:
PARASITOLOGIA
Year:
2009
Document type:
Article
Affiliation country:
Country of publication:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY