Detection of classical and atypical/Nor98 scrapie by the paraffin-embedded tissue blot method.
Vet Rec
; 164(22): 677-81, 2009 May 30.
Article
in En
| MEDLINE
| ID: mdl-19483208
ABSTRACT
The paraffin-embedded tissue (PET) blot method was used to investigate sections of the central nervous system and lymphatic tissues from 24 cases of classical scrapie and 25 cases of atypical/Nor98 scrapie in sheep and four healthy control sheep. The PET blot detected deposits of PrP(Sc) in the brain tissue of all 49 sheep with scrapie but no PrP(Sc) labelling could be detected in the control sheep. By contrast, not all the atypical/Nor98 scrapie cases were detectable by immunohistochemistry. The high sensitivity of the PET blot method made it possible to observe that in some atypical/Nor98 cases, deposits of PrP(Sc) may be restricted to supratentorial brain structures and that the diagnosis may be missed when only testing the obex area, where deposits are common in classical scrapie, and the cerebellar structures, where deposits are considered to be common in atypical/Nor98 cases.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Scrapie
/
Prions
/
Blotting, Western
/
Paraffin Embedding
Type of study:
Diagnostic_studies
/
Observational_studies
Limits:
Animals
Language:
En
Journal:
Vet Rec
Year:
2009
Document type:
Article
Affiliation country: