A new and efficient method for inhibition of RNA viruses by DNA interference.
FEBS J
; 276(16): 4372-80, 2009 Aug.
Article
in En
| MEDLINE
| ID: mdl-19614744
ABSTRACT
We report here a new method for inhibition of RNA viruses induced by dsDNA. We demonstrated that both long dsDNA molecules and short interfering DNA with a sequence complementary to that of viral RNA inhibited tobacco mosaic virus expression and prevented virus spread. Also, the expression of the HIV-1 gp41 gene in HeLa cells was inhibited by complementary short interfering DNA. We showed that Dicer processed dsDNA, which suggests activation of the cellular machinery involved in silencing of RNA. For the silencing of viral RNA effected with dsDNA, we coined the term DNA interference technology.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA Viruses
/
RNA, Viral
/
DNA, Antisense
/
Gene Silencing
/
Genes, Viral
Limits:
Humans
Language:
En
Journal:
FEBS J
Journal subject:
BIOQUIMICA
Year:
2009
Document type:
Article
Affiliation country: