Demethylation of promoter regulatory elements contributes to CD70 overexpression in CD4+ T cells from patients with subacute cutaneous lupus erythematosus.
Clin Exp Dermatol
; 35(4): 425-30, 2010 Jun.
Article
in En
| MEDLINE
| ID: mdl-19874375
ABSTRACT
BACKGROUND:
Impaired methylation of T-cell DNA is thought to contribute to the development of systemic lupus erythematosus (SLE). CD70 (TNFSF7) is a B-cell costimulatory molecule that contributes to excessive B-cell stimulation in vitro and in vivo. CD70 is overexpressed in CD4+ T cells of patients with SLE, and DNA demethylation occurs in promoter sequences that regulate CD70 expression in SLE CD4+ T cells. However, it is unknown whether the expression and methylation of CD70 in CD4+ T cells are affected in patients with subacute cutaneous lupus erythematosus (SCLE).OBJECTIVE:
To compare CD70 expression levels and the methylation status of the CD70 promoter region in CD4+ T cells from patients with SCLE and healthy controls.METHODS:
We used real-time RT-PCR to compare messenger RNA levels of CD70 and flow cytometry to compare CD70 protein levels in CD4+ T cells from patients with SCLE and healthy controls. Bisulphite sequencing was used to determine the methylation status of the CD70 promoter region.RESULTS:
CD70 is overexpressed at the surface of SCLE CD4+ T cells. Demethylation of the CD70 promoter region was seen in CD4+ T cells from patients with SCLE.CONCLUSIONS:
Demethylation of regulatory elements contributes to CD70 overexpression in CD4+ T cells of patients with SCLE.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lupus Erythematosus, Cutaneous
/
CD4-Positive T-Lymphocytes
/
CD27 Ligand
Limits:
Adult
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Female
/
Humans
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Male
/
Middle aged
Language:
En
Journal:
Clin Exp Dermatol
Year:
2010
Document type:
Article
Affiliation country: