Chromomycin A3 staining, sperm chromatin structure assay and hyaluronic acid binding assay as predictors for assisted reproductive outcome.
Reprod Biomed Online
; 19(5): 671-84, 2009 Nov.
Article
in En
| MEDLINE
| ID: mdl-20021715
ABSTRACT
Functional sperm tests such as the sperm chromatin structure assay (SCSA), chromomycin A3 staining (CMA(3)) and hyaluronic acid binding assay (HBA) have been suggested as predictive tests of fertility in vitro. This study aimed to define the clinical role of these functional parameters in assisted reproduction in a prospective cohort study. Conventional sperm diagnosis (motility, morphology and concentration) as well as SCSA, CMA(3) and HBA tests were performed on 205 semen samples [74 IVF, 94 ICSI and 37 combined IVF/intracytoplasmic sperm injection (ICSI)]. Main outcome parameters were fertilization rate, clinical pregnancy rate and take-home baby rate. The study showed that each of the three functional sperm tests was related to one or more conventional and one or more functional sperm tests, indicating that spermatozoa from patients with abnormal conventional semen parameters have a higher likelihood for multiple functional abnormalities. Only SCSA and CMA(3) staining were shown to have a limited predictive value when IVF or combined IVF/ICSI was applied. The proposed threshold value of
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Pregnancy Outcome
/
Chromatin
/
Chromomycin A3
/
Reproductive Techniques, Assisted
/
Fluorescent Dyes
/
Hyaluronic Acid
Type of study:
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Female
/
Humans
/
Male
/
Pregnancy
Language:
En
Journal:
Reprod Biomed Online
Journal subject:
MEDICINA REPRODUTIVA
Year:
2009
Document type:
Article
Affiliation country: