[Human papillomavirus (HPV) genotypes in cervix uterine cancer patients in a public hospital and private clinic from Santiago, Chile]. / Genotipos de virus papiloma humano (VPH) en pacientes con cáncer cervico-uterino en un hospital público y una clínica privada de Santiago, Chile.

ABSTRACT

We compared HPV genotypes among squamous cervical cancer samples from a public hospital (n = 55) and a private clinic (n = 35 cases) of Santiago. Paraffin-embedded specimens were analyzed by PCR followed by an immunoenzimatic assay. Reverse line blotting was used for the identification of 36 HPV genotypes. We found HPVDNAm 94.4% of all cancers. Single mfections HPV16 40.0%, (clinic 37.1%, hospital 41.8%) VPH187.8% (clinic 2.9%, hospital 10.9%); single+multiple mfections VPH16 61.1% (clinic 53.1%, hospital 71.7%), VPH18 34.4% (clinic 21.9%, hospital 45.2%). HPV16 orHPV18 occurredin 75.6% of cases, higher in the hospital than the clinic (87.3%-95% CI 84.9-96.3 - and 57. l%-95% CI 46.6-66 - respectively, p = 0.002). Other genotypes in single mfections HPV 26, 31, 33, 45, 58, 67; in co-mfections HPV 35,52,56,59 and 66. HPV16 but specially HPV 18 were significantly more frequent in the public hospital; 75.6% of squamous cervical cancer were associated to the vaccine preventable HPV16/18.