Coherent diffractive imaging of biological samples at synchrotron and free electron laser facilities.
J Biotechnol
; 149(4): 229-37, 2010 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-20149827
ABSTRACT
Coherent X-ray diffractive imaging (CXDI) is a new imaging technique that offers the potential to image non-crystalline materials to sub-nanometer resolutions. Here we review the progress in CXDI of biological samples at both synchrotron and free electron laser (FEL) sources. We outline the experimental design of a CXDI experiment and summarize the iterative phase retrieval techniques that are used to produce images from the measured diffraction patterns. We describe a selection of key experiments performed in bio-imaging with CXDI from synchrotron sources, and we discuss the proof-of-principle experiments performed at FLASH at DESY in Hamburg. Finally, we show through simulation that for realistic parameters of hard X-ray FELs a resolution of a few nanometers may be achieved for individual biological objects imaged with single pulses of FEL radiation. Furthermore, we revise how this resolution may be improved to the sub-nanometer range if we image multiple copies of samples with a reproducible structure.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
X-Ray Diffraction
/
Diagnostic Imaging
/
Synchrotrons
/
Lasers
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2010
Document type:
Article
Affiliation country: