Evaluation of a primer-probe energy transfer real-time PCR assay for detection of classical swine fever virus.
J Virol Methods
; 168(1-2): 259-61, 2010 Sep.
Article
in En
| MEDLINE
| ID: mdl-20471428
ABSTRACT
This study describes evaluation of a real-time PCR assay based on primer-probe energy transfer (PriProET) technology for detection of classical swine fever virus (CSFV). The PriProET technology allows melting curve analysis following PCR amplification and thus provides a higher specificity. The assay was compared with a TaqMan assay by testing a total of 203 samples including 175 clinical specimens and 28 batches of Hog Cholera Lapinized Virus (HCLV) vaccine. The two assays gave the same results for 184 (91%) samples. Compared with the TaqMan assay, 19 additional samples were found to be positive for CSFV using the PriProET assay. In an RNA mixture of both wild type CSFV and C-strain vaccine, the melting curves displayed only one curve either a wild type-like or a vaccine-like depending on the dominating RNA. The PriProET assay can be a routine molecular tool or a confirmative tool for diagnosis of classical swine fever (CSF), especially in the case of samples that yield an inconclusive result by the TaqMan assay.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virology
/
Oligonucleotide Probes
/
Polymerase Chain Reaction
/
DNA Primers
/
Classical Swine Fever
/
Classical Swine Fever Virus
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Animals
Language:
En
Journal:
J Virol Methods
Year:
2010
Document type:
Article
Affiliation country: