[Optimization of method of titration rabies virus with fluorescence focus units assay].
Zhongguo Yi Miao He Mian Yi
; 16(3): 258-60, 2010 Jun.
Article
in Zh
| MEDLINE
| ID: mdl-20726271
ABSTRACT
OBJECTIVE:
A new Fluorescence Focus Units (FFU) developed for Rabies Virus titration replace current reference method (LD50) so as to reduce the cost.METHODS:
The BSR cell was infected by triple serial dilution of the Rabies Virus CTN strain, then detect the fluorescent focus by FITC labeling anti-Rabies Virus monoclonal antibodies. All the samples were assayed in both FFU and LD50.RESULTS:
Data generated indicateed that a significant correlation between the two methods (r=99).CONCLUSION:
This method is simple and rapid. It will be considered as a useful alternative method to the LD50.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rabies
/
Rabies virus
/
Biological Assay
/
Fluorescent Antibody Technique
Type of study:
Evaluation_studies
Limits:
Animals
Language:
Zh
Journal:
Zhongguo Yi Miao He Mian Yi
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2010
Document type:
Article
Affiliation country: