Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus.
J Clin Microbiol
; 48(12): 4598-601, 2010 Dec.
Article
in En
| MEDLINE
| ID: mdl-20943869
ABSTRACT
The diagnostic sensitivities of the BD GeneOhm and Cepheid Xpert assays were compared using culture on log-serial dilutions of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and non-MRSA strains and on nasal and groin swabs from patients with histories of MRSA carriage. The sensitivities of GeneOhm and Xpert were high at 10(3)-CFU/ml MRSA concentrations (92.3% and 96.3%, respectively) although decreased considerably (<35%) at a 1-log-lower concentration. Unexpectedly, both assays also detected select coagulase-negative staphylococci, which requires further evaluation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Staphylococcal Infections
/
Carrier State
/
Bacteriological Techniques
/
Molecular Diagnostic Techniques
/
Methicillin-Resistant Staphylococcus aureus
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
J Clin Microbiol
Year:
2010
Document type:
Article
Affiliation country: