Conditions for the extraction of protease-free LMG proteins from calf thymus.
Biochem Int
; 22(5): 929-38, 1990 Dec.
Article
in En
| MEDLINE
| ID: mdl-2099153
ABSTRACT
Calf thymus LMG proteins are known to be susceptible to a wide variety of chromatin-associated proteases. We have investigated the use of different inhibitors to protect LMG chromosomal proteins from proteolytic degradation. In this work we have developed a preparative method for the extraction of LMG proteins from purified chromatin which provides an LMG protein extract devoid of proteolytic activity.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thymus Gland
/
Chromosomal Proteins, Non-Histone
Limits:
Animals
Language:
En
Journal:
Biochem Int
Year:
1990
Document type:
Article
Affiliation country: