Vitamin D3 treatment of Crohn's disease patients increases stimulated T cell IL-6 production and proliferation.
Aliment Pharmacol Ther
; 32(11-12): 1364-72, 2010 Dec.
Article
in En
| MEDLINE
| ID: mdl-21050239
ABSTRACT
BACKGROUND:
Vitamin D3 has shown immune-modulating effects in CD4+ T cells from Crohn's disease patients in vitro.AIM:
To investigate the effects of in vivo vitamin D3 treatment on T cells in Crohn's disease patients.METHODS:
Peripheral blood mononuclear cells (PBMC) were isolated at week 0 and at week 26 from 10 vitamin D3- and 10 placebo-treated Crohn's disease patients participating in a randomized, placebo-controlled, clinical trial study. Monocyte-depleted PBMC were stimulated with anti-CD3 and anti-CD28, and cultured for 7, days, to investigate CD4+ T-cell proliferation and T-cell cytokine production.RESULTS:
In vitamin D3-treated patients, the median 25-hydroxyvitamin D3 levels increased 70 nmol/L compared with -5 nmol/L in the placebo group. Vitamin D3 treatment increased interleukin-6 production (delta = 188 pg/mL, range -444 to 4071) compared with a decrease in the placebo group (delta = -896 pg/mL, range -3841 to 1323) (P < 0.02, Wilcoxon rank sum test). Interestingly, vitamin D3 increased the amount of proliferating stimulated CD4+ T cells from median 41% (range 10-75%) to 56% (range 26-77%) (P = 0.02, Wilcoxon rank sum test).CONCLUSIONS:
Vitamin D3 treatment of Crohn's disease patients increased the IL-6 levels. Interestingly, vitamin D3 treatment enhanced the CD4+ T cell proliferation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
T-Lymphocytes
/
Crohn Disease
/
Interleukin-6
/
Cholecalciferol
Type of study:
Clinical_trials
Limits:
Adult
/
Aged
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Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Aliment Pharmacol Ther
Journal subject:
FARMACOLOGIA
/
GASTROENTEROLOGIA
/
TERAPIA POR MEDICAMENTOS
Year:
2010
Document type:
Article
Affiliation country: