Immunohistochemical detection of arginine methylated proteins (MeRP) in archival tissues.
Histopathology
; 57(5): 725-33, 2010 Nov.
Article
in En
| MEDLINE
| ID: mdl-21083602
ABSTRACT
AIMS:
To (i) determine whether methylarginine-specific antibodies can be employed for standard immunohistochemical analysis of paraffin-embedded tissues, (ii) analyse methylarginine expression in normal and neoplastic tissues and (iii) correlate methylarginine expression with that of protein arginine methyltransferase (PRMT1), the predominant cellular arginine methyltransferase. METHODS ANDRESULTS:
Immunohistochemistry of normal and cancer tissues was performed utilizing three commercial polyclonal antibodies anti-methylarginine-specific antibody (anti-mRG) raised against a methylarginine peptide, Control antibody (anti-RG), a control antiserum raised against a corresponding arginine peptide without any methylated residues and anti-PRMT1. Nuclear and/or cytoplasmic methylarginine expression was detected in all keratinized and non-keratinized epithelia. A preliminary survey of a series of thyroid, pancreatic, colonic and gastric cancers identified a different pattern of methylarginine expression in comparison with normal tissue. A correlation between methylarginine staining and PRMT1 expression was found in all normal and cancer tissues analysed.CONCLUSION:
Methylarginine-specific antibodies are capable of recognizing methylarginine proteins (MeRP) in paraffin-embedded tissues. Methylarginine proteins are expressed widely and show differences in subcellular localization in various organs and neoplastic conditions. The efficient detection of methylproteins by standard immunohistochemistry provides a new tool to investigate the role of methylarginine proteins (MeRP) in biological processes including carcinogenesis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arginine
/
Immunohistochemistry
/
Protein Processing, Post-Translational
/
Neoplasms
Type of study:
Diagnostic_studies
Language:
En
Journal:
Histopathology
Year:
2010
Document type:
Article
Affiliation country: