The importance of exportin and Ran for nucleocytoplasmic shuttling of the ecdysteroid receptor.
Arch Insect Biochem Physiol
; 76(1): 12-21, 2011 Jan.
Article
in En
| MEDLINE
| ID: mdl-21125558
ABSTRACT
Nuclear localization of the ecdysteroid receptor (EcR) is increased in HeLa cells if exportin-1 (CRM1), a predominant carrier for export of proteins and RNA from the nucleus into the cytoplasm, is knocked down by siRNA against exportin. However, knockdown of the small G protein Ran, which is essential for nuclear transport, leads to an arrest of EcR in the cytoplasm, but does not prevent efficient nuclear import of the most important heterodimerization partner of EcR, ultraspiracle (Usp). Like in vertebrate cells, EcR is also distributed heterogeneously in Drosophila melanogaster S2 cells but shifted exclusively to the nucleus, if Usp is present.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Steroid
/
Receptors, Cytoplasmic and Nuclear
/
Ran GTP-Binding Protein
/
Karyopherins
/
Drosophila melanogaster
Limits:
Animals
/
Humans
Language:
En
Journal:
Arch Insect Biochem Physiol
Journal subject:
BIOLOGIA
/
BIOQUIMICA
Year:
2011
Document type:
Article
Affiliation country: