Effects of TNF-α and IL-1 ß on the activation of genes related to inflammatory, immune responses and cell death in immortalized human HaCat keratinocytes.
Int J Immunopathol Pharmacol
; 23(4): 1057-72, 2010.
Article
in En
| MEDLINE
| ID: mdl-21244755
ABSTRACT
The present experiments were designed to characterize by microarray analysis the transcriptional responses of human keratinocytes (HaCat) to TNF-α and IL-1 ß, given alone or in combination, in order to better understand the mechanisms underlying inflammatory, immune responses and cell death in which both cytokines play a pathophysiological role. Significant differences in the percentage and quality of genes dysregulated by TNF-α and IL-1 ß were shown. Both cytokines activated a series of genes involved in inflammatory, immune response as well as in cell death. In our experimental conditions, TNF-α, in contrast to IL-1 ß, did not induce a significant level of apoptosis in keratinocytes. However, given together both cytokines produced a significant decrease in apoptotic cells and synergistic transcriptional response which was due to the activation of several specific genes occurring after application of each cytokine. TNF-α and IL-1 ß evoked apoptotic effect and transcriptional responses were linked to the stimulation of their specific receptors since a pre-treatment with monoclonal antibodies vs TNF-α and/or IL-1 ß receptors was able to significantly reduce them.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Keratinocytes
/
Transcriptional Activation
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Gene Expression Profiling
/
Interleukin-1beta
Limits:
Humans
Language:
En
Journal:
Int J Immunopathol Pharmacol
Journal subject:
ALERGIA E IMUNOLOGIA
/
FARMACOLOGIA
/
PATOLOGIA
Year:
2010
Document type:
Article
Affiliation country: