Sox17-dependent gene expression and early heart and gut development in Sox17-deficient mouse embryos.
Int J Dev Biol
; 55(1): 45-58, 2011.
Article
in En
| MEDLINE
| ID: mdl-21305474
ABSTRACT
Sox17 is a transcription factor that is required for maintenance of the definitive endoderm in mouse embryos. By expression profiling of wild-type and mutant embryos and Sox17-overexpressing hepatoma cells, we identified genes with Sox17-dependent expression. Among the genes that were up-regulated in Sox17-null embryos and down-regulated by Sox17 expressing HepG2 cells is a set of genes that are expressed in the developing liver, suggesting that one function of Sox17 is the repression of liver gene expression, which is compatible with a role for Sox17 in maintaining the definitive endoderm in a progenitor state. Consistent with these findings, Sox17(-/-) cells display a diminished capacity to contribute to the definitive endoderm when transplanted into wild-type hosts. Analysis of gene ontology further revealed that many genes related to heart development were downregulated in Sox17-null embryos. This is associated with the defective development of the heart in the mutant embryos, which is accompanied by localised loss of Myocd-expressing cardiogenic progenitors and the malformation of the anterior intestinal portal.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Expression Regulation, Developmental
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HMGB Proteins
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Gastrointestinal Tract
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Embryo, Mammalian
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SOXF Transcription Factors
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Myocardium
Type of study:
Prognostic_studies
Limits:
Animals
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Female
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Humans
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Male
Language:
En
Journal:
Int J Dev Biol
Journal subject:
BIOLOGIA
/
EMBRIOLOGIA
Year:
2011
Document type:
Article
Affiliation country: