The CD133+ tumor stem-like cell-associated antigen may elicit highly intense immune responses against human malignant glioma.
J Neurooncol
; 105(2): 149-57, 2011 Nov.
Article
in En
| MEDLINE
| ID: mdl-21479962
ABSTRACT
To explore the immunogenicity of glioma stem-like cell-associated antigens (SAAs) from sorted or unsorted glioma tumor stem-like cells (TSCs) as well as irradiated TSCs. Two primary human malignant glioma lines (SHG62, SHG66) and U87 cell line were primarily cultured in the serum-free medium (SFM) supplemented with EGF/bFGF. TSCs were identified by their self-renewal, multi-lineage differentiation and tumorigenic activity. To prepare SAAs in vitro, CD133+ TSCs were sorted either by magnetic cell sorting or with irradiation (6 Gy).The cytotoxicity induced by autogenous myeloid dendritic cell (DC)-mediated SAA-specific cytotoxic T lymphocytes (CTLs) was assessed by the Just Another Method test. SHG62, SHG66, and U87 cells contained TSCs. CD133+ SAAs-specific CTLs were significantly more cytotoxic than effector cells loaded with unsorted SAA (P < 0.05). Effector cells loaded with irradiated SAAs were more cytotoxic than those with regular SAAs (P < 0.01). SAAs from CD133+ TSCs and irradiated TSCs provide highly immunogenic antigens. TSCs might be a novel source of antigens for DC vaccination against malignant gliomas.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Neoplastic Stem Cells
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Brain Neoplasms
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Glycoproteins
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T-Lymphocytes, Cytotoxic
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Antigens, CD
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Glioma
Type of study:
Risk_factors_studies
Limits:
Animals
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Female
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Humans
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Male
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Middle aged
Language:
En
Journal:
J Neurooncol
Year:
2011
Document type:
Article
Affiliation country: