The structural basis for the function of two anti-VEGF receptor 2 antibodies.
Structure
; 19(8): 1097-107, 2011 Aug 10.
Article
in En
| MEDLINE
| ID: mdl-21827946
ABSTRACT
The anti-VEGF receptor 2 antibody IMC-1121B is a promising antiangiogenic drug being tested for treatment of breast and gastric cancer. We have determined the structure of the 1121B Fab fragment in complex with domain 3 of VEGFR2, as well as the structure of a different neutralizing anti-VEGFR2 antibody, 6.64, also in complex with VEGFR2 domain 3. The two Fab fragments bind at opposite ends of VEGFR2 domain 3; 1121B directly blocks VEGF binding, whereas 6.64 may prevent receptor dimerization by perturbing the domain 3domain 4 interface. Mutagenesis reveals that residues essential for VEGF, 1121B, and 6.64 binding are nonoverlapping among the three contact patches.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Angiogenesis Inhibitors
/
Vascular Endothelial Growth Factor Receptor-2
/
Antibodies, Monoclonal
Type of study:
Prognostic_studies
Language:
En
Journal:
Structure
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
/
BIOTECNOLOGIA
Year:
2011
Document type:
Article
Affiliation country: