Presence and interaction of inflammatory cells in the spleen of Atlantic cod, Gadus morhua L., infected with Francisella noatunensis.
J Fish Dis
; 34(9): 687-99, 2011 Sep.
Article
in En
| MEDLINE
| ID: mdl-21838712
ABSTRACT
Serious infectious diseases, accompanied by macrophage-dominated chronic inflammation, are common in farmed Atlantic cod. To increase knowledge relating to morphological aspects of such inflammatory responses, cod were challenged with Francisella noatunensis, an important bacterial pathogen of this fish species. Tissue and cell dynamics in the spleen were examined sequentially over 60 days. Small clusters of mainly macrophage-like cells (MLCs) staining for non-specific esterase and acid phosphatase developed with time. These foci were transiently infiltrated by pleomorphic proliferating cells of unknown nature and by granulocyte-like cells (GCLCs) staining for peroxidase and lysozyme. The latter cell type, which appeared to be resident in the red pulp of control fish, migrated into the inflammatory foci of infected fish. Cells expressing genes encoding IFN-γ and IL-8 increased in number during the study period. Bacteria were detected only in the MLCs and their number increased despite the extensive inflammation. Our results demonstrate an intimate spatial relationship in inflammatory foci between at least three cell types. The presence of GCLCs, together with MLCs, suggests pyogranulomatous inflammation as a more appropriate descriptive term than granulomatous inflammation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spleen
/
Gram-Negative Bacterial Infections
/
Gadus morhua
/
Fish Diseases
/
Francisella
/
Inflammation
Limits:
Animals
Language:
En
Journal:
J Fish Dis
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
Year:
2011
Document type:
Article
Affiliation country: