Bioequivalence of topical clotrimazole formulations: an improved tape stripping method.
J Pharm Pharm Sci
; 14(3): 347-57, 2011.
Article
in En
| MEDLINE
| ID: mdl-21906479
ABSTRACT
PURPOSE:
Investigations were carried out to assess the use of tape stripping (TS) for the determination of bioequivalence of topical products containing 1% clotrimazole.METHODS:
The study design involved the establishment of an appropriate application time, which was determined by conducting a dose duration study. Subsequently, two bioequivalence studies were conducted i) using the brand (Canesten Topical - 1% clotrimazole cream) as both the test and the reference product and ii) comparing Canesten cream with a gel product containing the same concentration of clotrimazole (1%). Each tape strip was individually analyzed for clotrimazole content using an HPLC method and Transepidermal Water Loss (TEWL) measurements were used to normalize the stratum corneum thicknesses between subjects.RESULTS:
The results of the TS investigations showed that, if the study is sufficiently powered, tape stripping may be used to determine bioequivalence according to the conventional bioequivalence limits of 0.8-1.25, as well as detect formulation differences between different clotrimazole products.CONCLUSIONS:
The data from this study provided compelling evidence that tape stripping has the necessary attributes and potential to be used as a tool for the bioequivalence assessment of topical clotrimazole and/or other topical formulations, thereby circumventing the need to undertake expensive and time-consuming clinical trials for such products. This article is open to POST-PUBLICATION REVIEW. Registered readers (see "For Readers") may comment by clicking on ABSTRACT on the issue's contents page.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Clotrimazole
/
Dermatologic Agents
/
Surgical Tape
/
Antifungal Agents
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
En
Journal:
J Pharm Pharm Sci
Journal subject:
FARMACIA
/
FARMACOLOGIA
Year:
2011
Document type:
Article
Affiliation country: