Collagen matrix-bound clotting factors (CMBCF) promote healing-associated events independent of factor XIII in an in vitro model.

ABSTRACT

We have previously explored in vitro as well as in vivo models of the biological effects of liquid fibrin glue (FG) containing factor XIII. The fixed combination of a collagen matrix and coagulation factors I and IIa (TachoSil(®) , Nycomed, Linz, Austria) is void of factor XIII. We aimed to determine whether (1) this preparation exerts similar effects to liquid FG on cells in an in vitro system, or (2) this effect is modulated by factor XIII. In an in vitro model, the effect of the fixed combination of collagen matrix and coagulation factors I and IIa (collagen matrix-bound clotting factor [CMBCF]) on the expression and secretion of growth factors (vascular endothelial growth factor, platelet-derived growth factor, fibroblast growth factor-2) by gastric epithelial (AGS) and mesenchymal cells (fibroblasts), as well as their proliferative response (WST-test), was compared in the presence and absence of factor XIII. The use of CMBCF compared with collagen type I matrix resulted in an increased proliferation rate of fibroblasts; there was an increased secretion of fibroblast growth factor-2. Gastric epithelial cells secreted more vascular endothelial growth factor and platelet-derived growth factor into the culture supernatant in the presence of CMBCF. All responses remained unaltered by the addition of factor XIII in different concentrations. In conclusion, CMBCF exerted effects similar to liquid FG in an in vitro model of healing. The addition of factor XIII did not alter the response of mesenchymal or epithelial cells, with respect to proliferation and growth factor secretion.