A serological diagnostic survey for Brucella canis infection in Turkish patients with Brucellosis-like symptoms.
Jpn J Infect Dis
; 64(6): 516-9, 2011.
Article
in En
| MEDLINE
| ID: mdl-22116333
ABSTRACT
The incidence of Brucella canis infection in humans is unknown in Turkey. In this study, we investigated the prevalence of B. canis infection in human sera obtained from six regions in Turkey and comparatively evaluated the results obtained by agglutination-based techniques using standardized antigens made from B. canis. The patients (n = 1,746) presented with clinical symptoms that were similar to those of brucellosis. All patients who tested negative in the Rose Bengal test for the smooth Brucella strains (abortus, melitensis, and suis) were screened for evidence of B. canis infection using the rapid slide agglutination test (RSAT), the microagglutination test (MAT), and the 2-mercaptoethanol RSAT test (2ME-RSAT). Of the samples tested, 157 (8.9%), 68 (3.8%), and 66 (3.7%) were positive for B. canis, as determined by RSAT, MAT, and 2ME-RSAT, respectively. The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value of RSAT were 100%, 94.6%, 42%, and 100%, respectively, and of MAT were 100%, 99.9%, 97%, and 100%, respectively. We recommend the routine use of MAT and 2ME-RSAT to check the sera of all patients with symptoms of brucellosis who are negative for brucellosis using a smooth Brucella antigen.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brucellosis
/
Clinical Laboratory Techniques
/
Brucella canis
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prevalence_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Jpn J Infect Dis
Journal subject:
DOENCAS TRANSMISSIVEIS
Year:
2011
Document type:
Article