Regionalized Twist1 activity in the forelimb bud drives the morphogenesis of the proximal and preaxial skeleton.
Dev Biol
; 362(2): 132-40, 2012 Feb 15.
Article
in En
| MEDLINE
| ID: mdl-22178153
ABSTRACT
Development of the mouse forelimb bud depends on normal Twist1 activity. Global loss of Twist1 function before limb bud formation stops limb development and loss of Twist1 throughout the mesenchyme after limb bud initiation leads to polydactyly, the ulnarization or loss of the radius and malformations and reductions of the shoulder girdle. Here we show that conditional deletion of Twist1 by Mesp1-Cre in the mesoderm that migrates into the anterior-proximal part of the forelimb bud results in the development of supernumerary digits and carpals, the acquisition of ulna-like characteristics by the radius and malformations of the humerus and scapula. The mirror-like duplications and posteriorization of pre-axial tissues are preceded by disruptions to anterior-posterior Shh, Bmp and Fgf signaling gradients and dysregulation of transcription factors that regulate anterior-posterior limb patterning.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nuclear Proteins
/
Signal Transduction
/
Body Patterning
/
Twist-Related Protein 1
/
Forelimb
/
Morphogenesis
Limits:
Animals
Language:
En
Journal:
Dev Biol
Year:
2012
Document type:
Article
Affiliation country: