Differential cross-linking and radio-protective effects of genipin on mature bovine and human patella tendons.
Cell Tissue Bank
; 14(1): 21-32, 2013 Mar.
Article
in En
| MEDLINE
| ID: mdl-22350064
ABSTRACT
Gamma irradiation is a proven sterilization method, but is not widely used on allografts for anterior cruciate ligament reconstruction (e.g., patella tendon) due to radiation-induced decreases in mechanical strength. Addressing this drawback would improve the safety and supply of allografts to meet current and future demand. It was hypothesized that genipin-induced collagen cross-linking would increase the tensile modulus of patella tendon tissue such that 5 MRad gamma irradiation would not reduce the tissue mechanical strength below the original untreated values. Optimized genipin treatment increased the tensile modulus of bovine tendons by ~2.4-fold. After irradiation, genipin treated tissue did not significantly differ from native tissue, proving the hypothesis. Optimized genipin treatment of human tendons increased the tensile modulus by ~1.3-fold. After irradiation, both control and genipin-treated tissues possessed ~50-60% of their native tendon modulus, disproving the hypothesis. These results highlight possible age- and species- dependent effects of genipin cross-linking on tendon tissue. Cross-linking of human allografts may be beneficial only in younger donor tissues. Future research is warranted to better understand the mechanisms and applications of collagen cross-linking for clinical use.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Radiation-Protective Agents
/
Patellar Ligament
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Cross-Linking Reagents
/
Iridoids
Limits:
Animals
/
Female
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Humans
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Male
/
Middle aged
Language:
En
Journal:
Cell Tissue Bank
Journal subject:
HISTOLOGIA
/
TRANSPLANTE
Year:
2013
Document type:
Article
Affiliation country: