Preparation and characterization of biocompatible chitosan nanoparticles for targeted brain delivery of peptides.
Methods Mol Biol
; 846: 321-32, 2012.
Article
in En
| MEDLINE
| ID: mdl-22367822
ABSTRACT
Here, we describe a nanocarrier system that can transfer chitosan nanoparticles loaded with either small peptides such as the caspase inhibitor Z-DEVD-FMK or a large peptide like basic fibroblast growth factor across the blood-brain barrier. The nanoparticles are selectively directed to the brain and are not measurably taken up by liver and spleen. Intravital fluorescent microscopy provides an opportunity to study the penetration kinetics of nanoparticles loaded with fluorescent agents such as Nile red, and has demonstrated that this nanomedicine formulation is rapidly transported across the blood-brain barrier.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biocompatible Materials
/
Drug Delivery Systems
/
Chitosan
/
Nanoparticles
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2012
Document type:
Article
Affiliation country: