Measurement of cell death in Mammalian cells.
Curr Protoc Pharmacol
; Chapter 12: Unit12.8, 2012 Mar.
Article
in En
| MEDLINE
| ID: mdl-22382997
ABSTRACT
Methods for assessing mammalian cell death are presented in this unit. The unit is divided into six sections (1) a brief overview of cytotoxicity and pathways of cell death, (2) a method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity, (3) a flow cytometry method that simultaneously measures two types of cell death, necrosis, and apoptosis, (4) use of fluorescence microscopy and nuclear morphology to assess apoptosis and necrosis, (5) the use of multi-well plates and high-content analysis imaging systems to assess nuclear morphology, and (6) a discussion of the use of cytotoxicity assays to determine the mechanisms of cell death.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Death
/
Apoptosis
/
Toxicity Tests
/
Cell Culture Techniques
/
L-Lactate Dehydrogenase
/
Necrosis
Limits:
Animals
Language:
En
Journal:
Curr Protoc Pharmacol
Year:
2012
Document type:
Article
Affiliation country: