Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children.
Oral Surg Oral Med Oral Pathol Oral Radiol
; 113(3): 378-83, 2012 Mar.
Article
in En
| MEDLINE
| ID: mdl-22669144
ABSTRACT
OBJECTIVE:
The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children. STUDYDESIGN:
To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120.RESULTS:
Supernatants of all the C. albicans degraded type I collagen that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test P < .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test P > .05).CONCLUSIONS:
Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I collagen.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Candida albicans
/
HIV Infections
/
Collagen Type I
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Dental Caries
/
Mouth Mucosa
Limits:
Child
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Child, preschool
/
Female
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Humans
/
Male
Language:
En
Journal:
Oral Surg Oral Med Oral Pathol Oral Radiol
Year:
2012
Document type:
Article
Affiliation country: