Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning.
Biofouling
; 28(6): 563-9, 2012.
Article
in En
| MEDLINE
| ID: mdl-22694725
ABSTRACT
Doped diamond-like carbon (DLC) coatings offer potential antifouling surfaces against microbial and protein attachment. In particular, stainless steel surgical instruments are subject to tissue protein and resilient prion protein attachment, making decontamination methods used in sterile service departments ineffective, potentially increasing the risk of iatrogenic Creutzfeldt-Jakob disease during surgical procedures. This study examined the adsorption of proteins and prion-associated amyloid to doped DLC surfaces and the efficacy of commercial cleaning chemistries applied to these spiked surfaces, compared to titanium nitride coating and stainless steel. Surfaces inoculated with ME7-infected brain homogenate were visualised using SYPRO Ruby/Thioflavin T staining and modified epi-fluorescence microscopy before and after cleaning. Reduced protein and prion amyloid contamination was observed on the modified surfaces and subsequent decontamination efficacy improved. This highlights the potential for a new generation of coatings for surgical instruments to reduce the risk of iatrogenic CJD infection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Surgical Instruments
/
Prions
/
Decontamination
/
Creutzfeldt-Jakob Syndrome
/
Biofouling
/
Amyloid
Limits:
Animals
/
Humans
Language:
En
Journal:
Biofouling
Journal subject:
BIOLOGIA
Year:
2012
Document type:
Article
Affiliation country: