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Linear and conformational B cell epitope prediction of the HER 2 ECD-subdomain III by in silico methods.
Mahdavi, Manijeh; Mohabatkar, Hassan; Keyhanfar, Mehrnaz; Dehkordi, Abbas Jafarian; Rabbani, Mohammad.
Affiliation
  • Mahdavi M; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Asian Pac J Cancer Prev ; 13(7): 3053-9, 2012.
Article in En | MEDLINE | ID: mdl-22994709
ABSTRACT
Human epidermal growth factor receptor 2 (HER2) is a member of the epidermal growth factor receptor family of receptor tyrosine kinases that play important roles in all processes of cell development. Their overexpression is related to many cancers, including examples in the breast, ovaries and stomach. Anticancer therapies targeting the HER2 receptor have shown promise, and monoclonal antibodies against subdomains II and IV of the HER2 extra-cellular domain (ECD), Pertuzumab and Herceptin, are currently used in treatments for some types of breast cancers. Since anti HER2 antibodies targeting distinct epitopes have different biological effects on cancer cells; in this research linear and conformational B cell epitopes of HER2 ECD, subdomain III, were identified by bioinformatics analyses using a combination of linear B cell epitope prediction web servers such as ABCpred, BCPREDs, Bepired, Bcepred and Elliprro. Then, Discotope, CBtope and SUPERFICIAL software tools were employed for conformational B cell epitope prediction. In contrast to previously reported epitopes of HER2 ECD we predicted conformational B cell epitopes P1C 378-393 (PESFDGDPASNTAPLQ) and P2C 500-510 (PEDECVGEGLA) by the integrated strategy and and P4 PESFDGD-X-TAPLQ; P5 PESFDGDP X TAPLQ; P6 ESFDGDP X NTAPLQP; P7 PESFDGDP-X-NTAPLQ; P8 ESFDG-XX-TAPLQPEQL and P9 ESFDGDP- X-NTAPLQP by SUPERFICIAL software. These epitopes could be further used as peptide antigens to actively immune mice for development of new monoclonal antibodies and peptide cancer vaccines that target different epitopes or structural domains of HER2 ECD.
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Collection: 01-internacional Database: MEDLINE Main subject: Receptor, ErbB-2 / Epitopes, B-Lymphocyte Type of study: Prognostic_studies / Risk_factors_studies Limits: Animals / Humans Language: En Journal: Asian Pac J Cancer Prev Journal subject: NEOPLASIAS Year: 2012 Document type: Article Affiliation country:
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Collection: 01-internacional Database: MEDLINE Main subject: Receptor, ErbB-2 / Epitopes, B-Lymphocyte Type of study: Prognostic_studies / Risk_factors_studies Limits: Animals / Humans Language: En Journal: Asian Pac J Cancer Prev Journal subject: NEOPLASIAS Year: 2012 Document type: Article Affiliation country: