Suppressive effect of ascorbic acid on the mutagenesis induced by the bystander effect through mitochondrial function.
Free Radic Res
; 47(6-7): 474-9, 2013 Jul.
Article
in En
| MEDLINE
| ID: mdl-23560693
ABSTRACT
Here, we focused on suppressive effect of ascorbic acid (AsA) on changes in mitochondrial function and mutagenesis by the radiation- induced bystander effect (RIBE). In mammalian cell lines, medium transfer assay was performed and conditioned medium including secreted factors after X-irradiation were examined to detect the RIBE. We found that the membrane potential and increased levels of superoxide radical (O2(-)) in mitochondria were modulated in cells treated with conditioned medium from irradiated cells. The result of the present study also demonstrated that increases in reactive oxygen species (ROS) levels led to the induction of gene mutations. Interestingly, the modulations in mitochondria, in addition to mutation inductions by RIBE, were completely suppressed by treatment with AsA in cells treated with conditioned medium. These results suggest that mutagenesis, which may have resulted from secreted factors involving the RIBE, may be induced by ROS that are localized in mitochondria and may be relieved by AsA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ascorbic Acid
/
Mutagenesis
/
Bystander Effect
/
Mitochondria
Limits:
Animals
Language:
En
Journal:
Free Radic Res
Journal subject:
BIOQUIMICA
Year:
2013
Document type:
Article
Affiliation country: