Gene discovery in the tropical scallop Nodipecten nodosus: construction and sequencing of a normalized cDNA library.
Mar Environ Res
; 91: 34-40, 2013 Oct.
Article
in En
| MEDLINE
| ID: mdl-23669241
ABSTRACT
We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0 × 10(6) cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Library
/
Pectinidae
/
Genetic Association Studies
/
Genes
Limits:
Animals
Language:
En
Journal:
Mar Environ Res
Journal subject:
BIOLOGIA
/
SAUDE AMBIENTAL
/
TOXICOLOGIA
Year:
2013
Document type:
Article