Functional attributes of the Saccharomyces cerevisiae meiotic recombinase Dmc1.
DNA Repair (Amst)
; 12(9): 707-12, 2013 Sep.
Article
in En
| MEDLINE
| ID: mdl-23769192
ABSTRACT
The role of Dmc1 as a meiosis-specific general recombinase was first demonstrated in Saccharomyces cerevisiae. Progress in understanding the biochemical mechanism of ScDmc1 has been hampered by its tendency to form inactive aggregates. We have found that the inclusion of ATP during protein purification prevents Dmc1 aggregation. ScDmc1 so prepared is capable of forming D-loops and responsive to its accessory factors Rad54 and Rdh54. Negative staining electron microscopy and iterative helical real-space reconstruction revealed that the ScDmc1-ssDNA nucleoprotein filament harbors 6.5 protomers per turn with a pitch of â¼106Å. The ScDmc1 purification procedure and companion molecular analyses should facilitate future studies on this recombinase.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
Cell Cycle Proteins
/
Saccharomyces cerevisiae Proteins
/
DNA-Binding Proteins
Limits:
Humans
Language:
En
Journal:
DNA Repair (Amst)
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2013
Document type:
Article
Affiliation country: