The two-component system CBO2306/CBO2307 is important for cold adaptation of Clostridium botulinum ATCC 3502.
Int J Food Microbiol
; 167(1): 87-91, 2013 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-23810493
ABSTRACT
Clostridium botulinum is a notorious foodborne pathogen. Its ability to adapt to and grow at low temperatures is of interest for food safety. Two-component systems (TCSs) have been reported to be involved in cold-shock and growth at low temperatures. Here we show the importance of TCS CBO2306/CBO2307 in the cold-shock response of C. botulinum ATCC 3502. The relative expression levels of the cbo2306 and cbo2307 were up to 4.4-fold induced in the cold-shocked cultures but negatively regulated in the late-log and stationary growth phase in relation to early logarithmic growth phase in non-shocked cultures. Importance of the CBO2306/CBO2307 in the cold stress was further demonstrated by impaired growth of insertional cbo2306 or cbo2307 knockout mutants in relation to the wild-type strain ATCC 3502. The results suggest that the TCS CBO2306/CBO2307 is important for cold-shock response and adaptation of C. botulinum ATCC 3502 to low temperature.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adaptation, Physiological
/
Clostridium botulinum
/
Cold Temperature
/
Genes, Bacterial
Language:
En
Journal:
Int J Food Microbiol
Journal subject:
CIENCIAS DA NUTRICAO
/
MICROBIOLOGIA
Year:
2013
Document type:
Article