[Attenuation of cardiac ischemia/reperfusion injury by transient low hydroperoxide pretreatment in rat].

ABSTRACT

OBJECTIVE: To investigate how transient low dose of hydroperoxide pretreatment prevents cardiac ischemia/reperfusion injury. METHODS: SD rats were divided into 4 groups sham operation (Sham), standard ischemia/reperfusion (I/R), ischemic preconditioning (IPC) and IR preceded by low H2O2 treatment. Cardiac function and injury parameter were compared among groups. RESULTS: IPC protected reperfusion injury and improved cardiac function. Low H2O2 treatment played a role in cardioprotection similar to IPC. Low H2O2 was indeed generated in the early phase of simulated ischemia and attenuated cytochrome c release induced by high Ca2+ in isolated mitochondria. CONCLUSION: Low H2O2 plays a critical role in cardioprotection probably by inhibiting mitochondrial permeability transition.