Human mammospheres secrete hormone-regulated active extracellular vesicles.
PLoS One
; 9(1): e83955, 2014.
Article
in En
| MEDLINE
| ID: mdl-24404144
ABSTRACT
Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important prognostic factors for survival is the early detection of the disease. Recent studies indicate that extracellular vesicles may provide diagnostic information for cancer management. We demonstrate the secretion of extracellular vesicles by primary breast epithelial cells enriched for stem/progenitor cells cultured as mammospheres, in non-adherent conditions. Using a proteomic approach we identified proteins contained in these vesicles whose expression is affected by hormonal changes in the cellular environment. In addition, we showed that these vesicles are capable of promoting changes in expression levels of genes involved in epithelial-mesenchymal transition and stem cell markers. Our findings suggest that secreted extracellular vesicles could represent potential diagnostic and/or prognostic markers for breast cancer and support a role for extracellular vesicles in cancer progression.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mammary Glands, Human
/
Extracellular Space
/
Cell-Derived Microparticles
/
Hormones
Type of study:
Prognostic_studies
/
Screening_studies
Limits:
Female
/
Humans
Language:
En
Journal:
PLoS One
Journal subject:
CIENCIA
/
MEDICINA
Year:
2014
Document type:
Article
Affiliation country: